Structural Uniformity of Pullulan Produced by Several Strainsof Pullularia pullulans

Role of pH and nitrogen limitation in the elaboration of the extracellular polysaccharide pullulan by Pullularia pullulans.

During the growth of the yeastlike fungus Pullularia pullulans on glucose as sole carbon source, the cell-mass does not increase concomitantly with the elaboration of the extracellular polysaccharide pullulan, but precedes it. The conditions generated in the culture medium which activate the secretion of polysaccharide have been sought for, and, in particular, the roles of extracellular pH and ...

Pullulan Elaboration by Aureobasidium pullulans Protoplasts.

Protoplasts of Aureobasidium pullulans are capable of producing pullulan. Biosynthesis of the polymer pullulan required induction with kinetics similar to those of whole cells. The protoplasts also produced a heteropolysaccharide component containing mannose, glucose, and galactose. The relative proportions of the pullulan and heteropolysaccharide fractions were a function of glucose concentrat...

Uptake of glucose-l-14C by Pullularia pullulans.

Pullularia pullulans cells were grown in a medium containing yeast extract, malt extract, glucose, and nutrient salts, in addition to glucose-1-(14)C. The lipids extracted from the cells were fractionated by use of a single column packed with silicic acid. Of the total radioactive carbon added to the culture medium, the neutral lipid fractions contained 24.8%, whereas the phospholipid portions ...

CONTROL OF MOLECULAR WEIGHT DISTRIBUTION OF THE BIOPOLYMER PULLULAN PRODUCED BY THE FUNGUS Aureobasidium pullulans

Pullulan production from Aureobsidium pullulans by continuous culture

Ten isolates of Aureobasidium pullulans were screened for the highest production of pullulan and minimal melanin production from Fayoum Governorate, Egypt. The optimum conditions for continuous culture were studied; temperature was 24°C, while pH 5.0, and agitation speed was at 500 rpm, while the aeration rate was 2.5 vvm and the best flow rate was 1 L/ 24 h. The maximum production of pullulan ...